Characterization of Adipogenic  Differentiation of Mesenchymal Stem Cell  Derived from Mice Bone Marrow

Zahran F; El-Deen IM; Hamed S; EL-shenawy A

Volume : II, Issue : VI, June - 2013

Characterization of Adipogenic Differentiation of Mesenchymal Stem Cell Derived from Mice Bone Marrow

Zahran F, El Deen Im, Hamed S, El Shenawy A

Abstract :

Mesenchymal stem cells (MSCs) isolated from bone marrow (BM) possess the capacity for self-renewal and the potential for multilineage differentiation, in the present study we characterized the adipogenic differentiation of mesenchymal stem cells derived from C57BL/6 mice bone marrow. This is characterized by gene expression in different days (3, 8, 10, 14 days) during the process (C/EBP, PPAR-γ, LPL, FABP4, and B-Actin) which showed different stages of differentiation, although by stained the cells with Oil Red O stain which is specific for adipocyte and read absorbance for stained cells, so these results support that bone marrow from C57BL/6 give high yield of MSCs and show that there are specific genes for adipogenic differentiation which play vital role in differentiation.