Chemical composition and bioactivity of isolated components of Echinophora Platyloba, a medicinal plants find in Kurdistan region of Iraq, using GC–Ms, NMR and MTT bioassay method.

Baram AHMED HAMAH AMEEN

Volume : IV, Issue : X, October - 2015

Chemical composition and bioactivity of isolated components of Echinophora Platyloba, a medicinal plants find in Kurdistan region of Iraq, using GC–Ms, NMR and MTT bioassay method.

Baram Ahmed Hamah Ameen

Abstract :

In this study, a useful medicinal plant, Echinophora Platyloba ( known as Kushndar) find in Our region and used daily by many people as disordering, preserver and anti–lipid absorbent , has been studied. Extractions by different solvents have been carried out (using Methanol, Ethyl acetate and Hexan) it found that MeOH is best solvent for extraction (7.38%). More than 25 compounds determined by Gc–Mass–Mass technique and Elemental analysis shows high ratio of some useful elements ( Fe, Mn, Cu and Zn ) with (63150 ppb, 8756 ppb,1617 ppb and 1264 ppb respectively) in addition to Chromium( Cr 367.1 ppb) know as diabetic regulator element. Bioactivity (using ,MTT, 3–(4,5–dimethylthiazol–2–yl)–2,5–diphenyltetrazolium omide) for each extract shown that all of them has high activities ( MeOH extracts 0.2635, Ethyl acetate 0.3720 and hexane 0.526), Hence they need to be studied in order to find all active components for each parts by different separation methods such as (mplc, GC–Mass and TLC preparative) and finally identifying by NMR spectrophotometer.