Volume : II, Issue : II, February - 2013

Chemical Synthesis of Peptides

Tanaji Dnyanadev Padalkar

Abstract :

The aim of a peptide or a protein synthesis is the production of pure compounds of known structure. Common elements in any chemical synthesis of both peptides and proteins are the assembly of protected amino acids or peptide chains, their deprotection, purification and characterization. The basic strategy of SPPS still persists as the initial idea, outlined by Memfield. The process requires a solid insoluble support to scaffold the first amino acid residue and subsequent stages of the peptide as it is lengthened. The carboxyl end of the peptide is scaffolded by a covalent attachment to the polymeric support. The N-terminus needs protection and deprotection at each stage of the stepwise synthesis and resulting amino group should be the free -NH2 after each deprotection. The I^-protected amino acid is activated for coupling to the growing peptide chain. For a stepwise elongation of peptides on a polymeric support, these three steps, deprotection, neutralization and coupling would be repeated until the desired sequence is assembled. Finally the covalent anchoring bond would be cleaved to liberate the free peptide.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Tanaji Dnyanadev Padalkar Chemical Synthesis of Peptides Introduction, Process, Uses, Methods, Advantages. International Journal of Scientific Research, Vol.II, Issue.II February 2013

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