Volume : VII, Issue : VI, June - 2018

Comparative Analysis of ?-Hemolysin and ?-Hemolysin by HPLC and FT-IR.

Mohammed Azim Bagban, Dr. Nayan Jain

Abstract :

Hemolysins or haemolysins are lipids and proteins that cause lysis of red blood cells by destroying their cell memane.Most hemolysins are protein compounds, but others are lipids biosurfactants. α–hemolysin (α–toxin, Hla) is the prototype for the class of small β–barrel pore–forming cytotoxins (PFTs). α–toxin is secreted as a water soluble monomer, capable of binding and oligomerization into a heptameric structure on the host cell memane. β–Hemolysin is produced by specialized microorganisms and most readily identified by the characteristic zone of darkening or discoloration that it produces on sheep blood–agar plates.HPLC has revolutionized the efficiency and speed of separation of molecules in general and peptides in particular. An FTIR spectrometer simultaneously gathers high–spectral–resolution data over a wide spectral range. This confers a substantial benefit over dispersive spectrometer techniques. In the present study HPLC chromatograms and FT–IR spectra of both the toxins were compared for better enhancement of their study. 

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Mohammed Azim Bagban, Dr. Nayan Jain, Comparative Analysis of ?-Hemolysin and ?-Hemolysin by HPLC and FT-IR., INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : Volume-7 | Issue-6 | June-2018

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