COMPARATIVE ANTIBACTERIAL ASSESSMENT OF ANTIBIOTIC BASED IRRIGANTS AGAINST ENTEROCOCCUS FAECALIS - AN IN VITRO STUDY

Volume : VII, Issue : VI, June - 2018

Natarajan Senthil Nathan, Dhanasekaran Sihivahanan, Premkumar Elavarasu, Kingston Chellapandian, Deepak

AIM:The aim of the study is to compare the efficacy of 4 different generations of cephalosporins as an irrigant in eliminating E.faecalis by various

antibacterial assays such as disc diffusion assay, tube dilution assay and finally colony forming units

METHODS:Sixty intact lower premolar teeth were selected for this study. The tooth was decoronated below cementoenamel junction. Each

specimen was individually packed in vials containing 5ml of BHI oth. The specimens were autoclaved at 121˚c for 15 lbs for 15 minutes

A total of 60 specimens were divided into 6 groups. The specimens were placed in 5 ml of the sterile BHI oth belonging to groups were chosen for

contamination with Enterococcus faecalis. From each vial 2ml of sterile BHI oth was discarded and replaced with 2ml of Enterococcus faecalis

suspension prepared as stated previously under sterile conditions. The inoculated vials were incubated at 37˚C for 14 days, each of the tooth

specimens was taken from each vial under sterile conditions and was rinsed with 5ml of sterile saline solution. 0.5% of the irrigating solutions were

inserted into the canal with sterile syringes and 27 gauge needle. The irrigation solution was allowed to remain for 10 minutes after with they were

removed using sterile paper points. The samples were diluted using sterile saline. 100μl of sample from each dilutions was spread out on to the

surface of BHI agar plates. The plates were incubated at 37˚C for an overnight period. After incubation the colonies were counted and expressed as

CFU/ML.

RESULTS:The results of the present study show group 2 (cefuroxime) with least antimicrobial activity against Enterococcus faecalis. Whereas,

group 3 (cefoperazone) had better antibacterial activity compared to group 2 (cefuroxime) but had less antimictobial property when compared to

group 1 (cephazolin) and group 4 (cefipime). Group 1 (cephzolin) had better antimicrobial property when compared with group 2 (cefuroxime) and

group 3 (cefoperazone). Group 4 (cefipime) had better inhibition of bacterial growth of all other groups used in this experiment.