Volume : V, Issue : IV, April - 2016

COMPARATIVE EVALUATION OF CONVENTIONAL TECHNIQUES; MGIT AND REAL TIME PCR FOR THE DIAGNOSIS OF MYCOBACTERIUM TUBERCULOSIS FROM VARIOUS CLINICAL SAMPLES

Deepshikha Mangat, Kanwardeep Singh, Nirmal Kajal, Pushpa Devi

Abstract :

 The objective of the study was to Isolate & characterize Mycobacterium tuberculosis from pulmonary and extrapulmonary clinical samples & to standardize Real Time PCR for detection of M.tuberculosis in various clinical samples & to study utility of PCR over conventional methods for the diagnosis of tuberculosis. Various diagnostic modalities were also compared for their sensitivity, specificity & turnaround time. This study comprised of one hundred samples taken from clinically suspected cases of tuberculosis. Specimens from suspected cases were processed for Ziehl–Neelsen (ZN) stain and culture for M. tuberculosis was performed on Lowenstein–Jensen (LJ) medium as well as the BACTEC MGIT 320 system. All the samples were processed for PCR amplification with primers targeting 123 bp fragment of insertion element IS6110 sequence of M. tuberculosis complex using Taq Man chemistry. These methods were then compared for their rate of isolation. Out of 74 pulmonary samples, 32.75% were positive on ZN, 39.65% on LJ, 72.41% on MGIT and 94.82% on Real time PCR. LJ & MGIT negative and smear, LJ & MGIT negative. PCR was found to be 100% specific. None of the conventional methods combine a reasonable sensitivity with time to detection as real time PCR does. Even though these methodologies might be expensive for developing countries, the cost–benefit of this test must be considered. It is less expensive than a prolonged patient stay in the hospital wards, which is common for tuberculosis patients. The most important feature of Real Time detection chemistry is its high sensitivity for smear–negative specimens.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Deepshikha Mangat, Kanwardeep Singh, Nirmal Kajal, Pushpa Devi COMPARATIVE EVALUATION OF CONVENTIONAL TECHNIQUES; MGIT AND REAL TIME PCR FOR THE DIAGNOSIS OF MYCOBACTERIUM TUBERCULOSIS FROM VARIOUS CLINICAL SAMPLES International Journal of Scientific Research, Vol : 5, Issue : 4 APRIL 2016

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