Volume : VII, Issue : XI, November - 2018

COMPARATIVE EVALUATION OF NEEM EXTRACT,LEMON GRASS EXTRACT AND SODIUM HYPOCHLORITE AS AN IRRIGANT AGAINST ENTERCOCCUS FAECALIS,STERTOCOCCUS MUTANS AND LACTOBACILLUS ACIDOPHILUS- AN INVITRO STUDY

Annapoorna Kini, Chaitra J, Geetha Ib

Abstract :

 

The aim of this study was to evaluate the antimicrobial activity of auxiliary chemical substances  and natural extracts  such as neem extract ,lemon grass oil and sodium hypochlorite on lactobacillus ,streptococcus mutans  and Enterococcus faecalis inoculated in root canals

MATERIALS AND METHOD

 Antibacterial efficacy of neem extract and citronella oil was evaluated using sodium hypochlorite and physiological saline as control on Lactobacillus acidophilus, Streptococcus mutans and Enterococcus faecalis by plate assay with well diffusion technique.A comparative bioactivity–guided fractionation of two medicinal plants and sodium hypochlorite  was carried out using solvents with different  concentrations in methanol. Cultures were spread over the surface of the plate, and wells were punched out in every plate. Each well was inoculated by each extract and incubated for 24 h at 37 0 C and the zone of inhibition around each well was recorded.. Data presented as mean and standard deviation values. One–way analysis of variance (ANOVA) was used for comparisons between more than two groups. Tukeys post–hoc test was used for pair–wise comparison between the groups when ANOVA test was significant.

RESULT: Sodium hypochlorite was taken as a positive control, for comp the efficacy of the herbal extracts. Neem extract manifested  similar antimicrobial efficacy like  Sodium hypochlorite. Sodium hypochlorite showed good zone of inhibition against all selected microorganisms.

Conclusion: In the current study comparative analysis of the antibacterial efficacy of lemongrass oil and neem extract was made with Sodium hypochlorite and physiological saline as controls against Enterococcus faecalis, Lactobacillus acidophilus and Streptococcus mutans. Neem extract showed better results compared to the lemongrass oil. Hence neem extract can be used as root canal irrigant. Outcomes of the study indicated the efficacy of neem extract as better than the controls.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

COMPARATIVE EVALUATION OF NEEM EXTRACT,LEMON GRASS EXTRACT AND SODIUM HYPOCHLORITE AS AN IRRIGANT AGAINST ENTERCOCCUS FAECALIS,STERTOCOCCUS MUTANS AND LACTOBACILLUS ACIDOPHILUS- AN INVITRO STUDY , ANNAPOORNA KINI, CHAITRA J, GEETHA IB , INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : Volume-7|Issue-11| November-2018


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