Comparative study of immunoassay and Gas chromatography for chlorpyrifos detection in laboratory spiked water samples

Prabha parmar; Devinder Kocher

Volume : VI, Issue : II, February - 2017

Comparative study of immunoassay and Gas chromatography for chlorpyrifos detection in laboratory spiked water samples

Prabha Parmar, Devinder Kocher

Abstract :

Indirect and competitive enzyme–linked immunosorbent assay (I–ELISA and C–ELISA) were developed optimized and compared with gas chromatography (GC) for chlorpyrifos (CPF), detection. Chlorpyrifos (haptens) was conjugated to BSA proteins to obtain immunogens. Cross–reactivity (CR) for CPF was found less than 20 % for I–ELISA while showed no CR for BSA. Characterization for limit of detection was done against chlorpyrifos residue which reveals GC was the fastest and most sensitive mehod with the LOD of 0.1 ng ml⁻¹ while C–ELISA and I–ELISA shows LOD of 100 ng ml⁻¹and10 ng ml⁻¹ respectively. All methods were further applied to analyzed % recoveries for chlorpyrifos spiked samples and mean recovery found to be between 85% to110% and comparable LOD�S for all three techniques thereby immunoassay can be used for screening of food and environmental samples for CPF residues without complicated clean–up as in case of GC.