Comparison Between Total Leukocyte Cell  Count From Flowcytometer and Corrected  Total Nucleated Cell Count from A Dual  Platform Analysis

S  Mohammed Adil; R Muthukumar; P Jaimurugan; S Shankar; M Alagupandian; P Chirayu

Volume : II, Issue : X, October - 2013

Comparison Between Total Leukocyte Cell Count From Flowcytometer and Corrected Total Nucleated Cell Count from A Dual Platform Analysis

S Mohammed Adil, R Muthukumar, P Jaimurugan, S Shankar, M Alagupandian, P Chirayu

Abstract :

Umbilical cord blood is an alternate for bone marrow in stem cell transplantation. The treatment efficacy depends on total nucleated cell count (TNCC) and hematopoietic stem cells (HSC) count in the cord blood. Hence nucleated red blood cells (NRBC) enumeration, for quantifying corrected TNCC (cTNCC) becomes important for accurate prediction of clinical transplantation outcome. The objective of this study is to confirm that the total leukocyte cell count (TLCC) is equivalent to cTNCC. Methods: 20 cord blood samples are used to validate cTNCC obtained from dual platform analysis is equivalent to TLCC obtained using Flowcytometer. TNCC was obtained from hematology analyzer, whereas NRBC and TLCC from flowcytometer. Results: Statistical significance identified using Passing and Bablok fit showed a very high level of agreement between cTNCC and TLCC Conclusion: TLCC from flowcytometer can be safely used to report nucleated cell count of cord blood units instead of cTNCC. Abeviations: UCB – Umbilical cord blood; HSC – Hematopoitic Stem cells; PB – Peripheral blood; HLA –Human Leukocyte antigen; TNC – Total Nucleated Cells; TLC – Total Leukocyte cells; NRBC – Nucleated Red Blood Cells; TNCC – Total Nucleated Cell Count; TLCC – Total Leukocyte Cell Count; cTNCC – Corrected Total Nucleated Cell Count; ISHAGE – International Society for Hematotherapy and Graft Engineering