Comparison of Culture and Antigen Detection Method from Cerebrospinal Fluid for Diagnosis of Acute Pyogenic Meningitis due to Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae type b in a Tertiary Care Hospital

Bhakare Prashant; Shinde Alka N; De Anuradha S; Baveja Sujata M

Volume : V, Issue : VIII, August - 2016

Comparison of Culture and Antigen Detection Method from Cerebrospinal Fluid for Diagnosis of Acute Pyogenic Meningitis due to Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae type b in a Tertiary Care Hospital

Bhakare Prashant, Shinde Alka N, De Anuradha S, Baveja Sujata M

Abstract :

Objectives: This study was intended to isolate and identify and do antigen detection test for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae type b from CSF samples in acute pyogenic meningitis.Material & Methods: Fifty CSF samples from patients with acute pyogenic meningitis were cultured and identified as per standard techniques. Antigen detection was done by using PASTOREX MENINGITIS kit (Bio–Rad France).Results: Majority were adults (64%), maximum were in the age group of 16–40 years (38 %). Only three Streptococcus pneumoniae grew in CSF samples but Hemophilus influenzae and Neisseria meningitidis did not grow. Antigen detection test positivity was 30%, maximum being Streptococcus pneumoniae (16%), followed by Neisseria meningitidis (10%) and Hemophilus influenzae (4%). It was significant than culture (p=0.002).Conclusion: Antigen detection test of CSF sample is superior to culture in diagnosis of acute pyogenic meningitis, is rapid, easy to perform with not much expertise.