COMPARITIVE STUDY ON PHENOTYPIC CARBAPENEMASE ENZYME DETECTION METHODS AMONG ENTEROBACTERIACEAE CLINICAL ISOLATES IN A TERTIARY CARE HOSPITAL

Dr.Radhika Katragadda; Dr.G.Purushothaman

Volume : VIII, Issue : VII, July - 2019

COMPARITIVE STUDY ON PHENOTYPIC CARBAPENEMASE ENZYME DETECTION METHODS AMONG ENTEROBACTERIACEAE CLINICAL ISOLATES IN A TERTIARY CARE HOSPITAL

Dr. Radhika Katragadda, Dr. G. Purushothaman

Abstract :

The emergence of carbapenem resistance in Enterobacteriaceae is increasingly reported all over the world. Early detection of these carbapenem resistant Enterobacteriaceae (CRE) is mandatory to contain the spread of these drug resistant organisms. This study is aimed at identifying the efficacy of phenotypic methods in detecting carbapenemase production and to compare it with genotypic method. 50 Gram Negative Enterobacteriaceae isolates showing resistance to meropenem, were tested for carbapenemase production by Modified Hodge test (MHT), Combined Imipenem–EDTA disk test and Modified carbapenemase inactivation method (mCIM) and also tested for KPC, NDM, OXA–48 genes by PCR method. Among the 50 isolates, 41 isolates demonstrated drug resistance genes by genotypic method. Remaining 9 isolates were negative for carbapenemase production by both phenotypic and genotypic method. Among the 41 isolates, 20(48.78%) were MHT positive, 21 (51.21%) were Combined Imipenem–EDTA disk test positive, 3 isolates were positive for both MHT and Combined Imipenem–EDTA disk test and 40isolates (97.56%) were positive for mCIM.This study shows that the mCIM is specific, reliable and inexpensive phenotypic method to detect carbapenemase producing Enterobacteriaceae