Conservation and in Vitro Mass Multiplication  of Endangered Medicinal Plant, Leptadenia  Reticulata (Retz.) Wight & Arn. Through  Nodal Explant

Poonam Dhawan; Shilpi Damor

Volume : II, Issue : X, October - 2013

Conservation and in Vitro Mass Multiplication of Endangered Medicinal Plant, Leptadenia Reticulata (Retz.) Wight & Arn. Through Nodal Explant

Poonam Dhawan, Shilpi Damor

Abstract :

An efficient protocol has been developed for plant regeneration from nodal explants of Leptadenia Reticulata (Retz.) Wight and Arn. The population of Leptadenia reticulata (Retz.) Wight and Arn are not sustaining as they cannot reproduce fast enough in the wild to keep up with the demand. Unsustainable extraction, multiple uses and hence the huge demand for this plant by the traditional medicine and industries make this plant endangered. There is a need for development of alternative methods for quick and efficient propagation, for conservation and utilisation of germplasm of Leptadenia reticulata (Retz.) Wight & Arn. A range of various auxins and cytokinins with different concentrations and effect of various media have been investigated for multiple shoot induction using nodes, internodes and shoot tips as explants. The best results were obtained by nodal segment cultured on MS medium fortified with NAA (1.00mg/l) with BAP (4.0mg/l) .The regenerated shoots rooted best on full strength MS medium containing IBA (2.0mg/l). Regenerated plantlets with well–developed shoots and roots were hardened, successfully transferred to soil and maintained in green house. The present in vitro procedure can be used in conservation and mass propagation of this endangered medicinal plant