CYTOTOXICITY OF A NEWER RESIN CEMENT COMPARED TO GLASS IONOMER AND MINERAL TRIOXIDE AGGREGATE AN  IN–VITRO STUDY

LITTY VARGHESE; Prof.(Dr.)Aditya Shetty; Prof .(Dr.) Mithra .N. Hegde

Volume : V, Issue : I, January - 2016

CYTOTOXICITY OF A NEWER RESIN CEMENT COMPARED TO GLASS IONOMER AND MINERAL TRIOXIDE AGGREGATE AN IN–VITRO STUDY

Litty Varghese, Prof. Dr. Aditya Shetty, Prof . Dr. Mithra . N. Hegde

Abstract :

OBJECTIVE: The aim of the study was to compare and to check the cytotoxicity of a newer resin cement neoprene with and without addition of barium to that of mineral trioxide aggregate and glass inomer cement. MATERIALS AND METHODS: Growth and maintenance of human gingival fioblast was done in Dulbecco�s modified eagle�s medium (DMEM) the test samples were divided into four groups and the elute of the test materials were made in contact with cells and also the resin cement with and without addition of barium sulphate was serially diluted uptp 1:5 and MTT assay was performed for 24 hrs and 48 hrs of contact with cells . cells with DMEM was set as control group Statistical analysis was done using one way –ANOVA test Tukey�s HSD test RESULTS: Results showed that resin cement with addition of barium sulphate showed the highest viability followed by neoprene ,GIC and MTA. No statistical significant result in both serially diluted resin cements. CONCLUSION: In this study extracts of neoprene with barium sulphate had the highest viability followed by neoprene , GIC and MTA. statistically significant result were seen between the cells exposed to control group and cells exposed to MTA and also between the extract of neoprene with barium sulphate and MTA