Defensive role of Diazepam against sleep deprived behaviour

Dr. Md Shadab Alam; Dr. Neelam Agrawal; Dr. Arun Kumar Choudhary; Dr. Satish Chandra

Volume : VIII, Issue : II, February - 2019

Defensive role of Diazepam against sleep deprived behaviour

Dr. Md Shadab Alam, Dr. Neelam Agrawal, Dr. Arun Kumar Choudhary, Dr. Satish Chandra

Abstract :

The aim of this study was to explore the defensive role diazepam against stress produced as a result of sleep deprivation in albino mice. So, in this study healthy male albino mice weighing between 25–30 grams were divided into three groups with six animals in each group. The first group A were represented as negative control, i.e. (without sleep deprivation), second group B were represented as positive control and subjected to induce 48 hours of sleep deprivation (by placing on a grid suspended over water, based on modified method of Shinomiya et al.) Whereas, the study group C was sleep deprived and administered diazepam to strength of 0.02mg/ml, by dissolving 5mg tab in 250ml of gum acacia orally once daily for 24 days. The defensive role of diazepam was evaluated by measuring anxiety level or stress produced as a result of sleep deprivation by Elevated plus maze model.