Demonstration and characterization of stem cells in pulp tissue of human third molar teeth – an ex – vivo study

Dr. Gauri Kokane; Dr. Srivalli Natarajan

Volume : VI, Issue : XII, December - 2017

Demonstration and characterization of stem cells in pulp tissue of human third molar teeth – an ex – vivo study

Dr. Gauri Kokane, Dr. Srivalli Natarajan

Abstract :

Aims and Objective– Aim of the study was isolation, demonstration and characterization of stem cells from the pulp tissue derived from human third molar teeth that are extracted for therapeutic purposes.

Materials and methods–

Study was carried out from December 2013 to August 2015 in the Department of Oral and Maxillofacial surgery, MGM Dental College and Hospital. 10 Samples were selected as per the inclusion and exclusion criteria and patients were explained about the study. Pulp tissue was retrieved from the samples and processed to achieve single cell suspension. Smears prepared from the single cell suspensions were processed for H and E stating and Immunocytochemistry (using Primary antibodies against CD44, CD133, Oct–4 and SSEA–4).

Results–

Significant number of samples demonstrated stem cells by H and E staining. Significant numbers of cells were positive for immunocytochemistry (ICC) by CD44, CD133 and Oct–4; while the expression of SSEA–4 was not clinically significant. All the P values of <0.05 were considered clinically significant.

Conclusion–

Human pulp tissue is a viable source of stem cells, which is easily accessible and from which these stem cells can be isolated with minimum infrastructure and minimum morbidity. Immunocytochemical studies confirm the presence and coexistence of Dental Pulp Mesenchymal Stem cells (DPMSC�s) and Dental Pulp Pluripotent stem cells (DPPSC�s) in the pulp tissue from human third molar teeth.