Detection of blaNDM-1 gene in multidrug resistant Escherichia coli in a tertiary care hospital of Punjab-North west India

SHAVETA KATARIA; SHIVANI; SHIPRA GALHOTRA; NEERJA JINDAL; DEEPAK ARORA; SHILPA ARORA; VISHAL SHARMA; PRAGATI GROVER; NITIKA DHURIA; HOBINDER ARORA; TRIMAAN KAUR BIANS; ANATO K SWU

Volume : IX, Issue : VIII, August - 2020

Detection of blaNDM-1 gene in multidrug resistant Escherichia coli in a tertiary care hospital of Punjab-North west India

Shaveta Kataria, Shivani, Shipra Galhotra, Neerja Jindal, Deepak Arora, Shilpa Arora, Vishal Sharma, Pragati Grover, Nitika Dhuria, Hobinder Arora, Trimaan Kaur Bians, Anato K Swu

Abstract :

INTRODUCTION– Escherichia coli, a common pathogen usually associated with urinary tract infections and diarrhoea, is becoming a major concern among hospital acquired infections. The exact proportion of NDM–1 E. coli in different parts of our country is still not known. Hence, the present study was undertaken to determine the occurrence of multidrug resistant and blaNDM–1 positive E. coli among the hospitalised patients and to study the demographic and clinical characteristics of the patients infected with blaNDM–1 positive E. coli. METHODOLOGY– The present hospital based descriptive study was conducted on 260 gram negative bacilli isolated from 5307 consecutive clinical specimens. The ief history and sociodemographic profile of the patients was recorded. The antibiotic susceptibility testing of E. coli isolates was performed by Kirby Bauer disk diffusion method and MIC values were obtained by VITEK2 Compact system. All the multidrug resistant E. coli which showed reduced susceptibility to carbapenems were subjected to Modified Hodge test (the confirmation of carbapenemase production), Combined disk synergy test (metallo–β–lactamase production) and blaNDM–1 gene by PCR. RESULTS– Of 260 gram negative bacilli, 129 were E. coli and 77(59.7%) were multidrug resistant. Eleven(8.5%) isolates were screened as resistant to carbapenem on Kirby Baeur Disc Diffusion method and 8(6.2%) demonstrated the presence of blaNDM–1 gene on PCR, while 8 were positive in MHT and 7 in CDST. Sociodemographic and clinical characteristics of patients having blaNDM–1 positive E. coli infections showed that these isolates infects patients of all age groups and factors like gender, geographical distribution, education status of the patients play statistically insignificant role in the acquisition of this infection. CONCLUSIONS– The present study underscores the need of early detection which would help to prevent the dissemination of NDM–1 positive E. coli by implementation of strict infection control measures, formulation of antibiotic policy and use of antibiotic stewardship program.