Detection of dengue infection by Rapid IgG/IgM SD Bioline card test.

Haya Khan; Jitendra Tiwari; Inderpal Soni; Bharti Malhotra

Volume : V, Issue : IX, September - 2016

Detection of dengue infection by Rapid IgG/IgM SD Bioline card test.

Haya Khan, Jitendra Tiwari, Inderpal Soni, Bharti Malhotra

Abstract :

Background and Objectives: Dengue virus (DENV) is the causative agent of a wide spectrum of clinical manifestations ranging from mild asymptomatic illness to severe fatal dengue haemorrhagic fever/dengue shock syndrome (DHF/DSS). The present study was carried out for rapid diagnosis of dengue infection in children using Dengue Rapid IgM/ IgG detection kit. Materials and Methods: Serum samples from 58 children <15 yrs of age clinically suspected of having dengue infection and admitted in J K Lone Hospital, Jaipur during the period of two months from November 2011 to December 2011, were tested for the presence of Dengue IgM and IgG antibodies using one–step immunochromatographic assay (Dengue Rapid IgG/IgM Test by SD BIOLINE Results: In 15/58 (25.80%) samples, both IgM and IgG antibodies were detected. In 3/58 (5.1%) samples only IgM antibodies were detected whereas in 19/58 (32.70%) samples, only IgG antibodies were detected. In 21/58 (36.20%) samples, none of the antibodies were detected. In total 18/58 (31.30%) samples, IgM antibodies were detected while IgG antibodies were detected in total 34/58 (58.60%) samples. Conclusion: Rapid immunochromatographic tests for IgG and IgM offer a convenient method to screen samples for dengue infection in field during epidemic threats. However confirmatory tests are also needed to be done for the confirmation of infection as specificity and sensitivity of these tests has yet to be verified.