Detection of ESBL-producing Escherichia Coli and Klebsiella pneumoniae in Patient Suffering with Symptomatic Urinary tract infection in Tertiary care Hospital.

Anil Kumar; Sushil Kumar

Volume : VII, Issue : VII, July - 2018

Detection of ESBL-producing Escherichia Coli and Klebsiella pneumoniae in Patient Suffering with Symptomatic Urinary tract infection in Tertiary care Hospital.

Anil Kumar, Sushil Kumar

Abstract :

Aim and objectives:This study is to determine the prevalence of Extended spectrum beta–lactamases (ESBLs)in urinary isolates. To compare different phenotypic methods for ESBL detection and to evaluate the antibiotic resistance patterns among ESBL producing urinary Escherichia coli.Methodology:Urinary isolates of E.coli that were resistant to atleast one of 3^rd generation cephalosporins (cefotaxime,ceftazidime) were screened and tested for ESBL production using the Double disc diffusion test (DDDT), quantitative E–strip method and Automated Vitek–2.Result:A total of 341 Klebsiellapneumoniae and Escherichia coli isolates were screened from patients with symptomatic UTI. 207 (60.7%)Escherichia coli, 134 (39.2%) Klebsiellapneumonia. ESBL production was detected in 48.8% and for 161 screening test positive E. coli, ESBL production was detected in 40%.