Volume : IV, Issue : VII, July - 2015

Detection of FLT3–ITD mutations and prognostic impact of NPM1 exon 12 mutations in adult CN–AML patients

Nada Tajuddin, Shadenmuawia, Amanifouadsorour, Khalid Bassiouny Mohamed

Abstract :

<p> Acute myeloid leukemia (AML) is a heterogeneous group of leukemias that arise in precursors of myeloid, erythroid, megakaryocytic, and monocytic cell lineages. These leukemias result from clonal transformation of hematopoietic precursors through the acquisition of chromosomal rearrangements and multiple gene mutations .In the past few years, mutations in genes, such as Fms–like tyrosine kinase 3 (FLT3) , the myeloid–lymphoid or mixed–lineage leukemia gene(MLL), CCAAT/enhancer binding protein alpha (CEBPA), and Nucleophosmin (NPM1), have been identified in Cytogenetically Normal–Acute Myeloid Leukemia (CN–AML), and the presence of such mutations carries prognostic information. We aimed to study the prevalence, association with FLT3–ITD mutations, and prognostic impact of NPM1, exon 12 mutations in 50adult AMLpatients with normal karyotype receiving induction chemotherapy (cytarabine and daunorubicin). We performed HRM assay to detect NPM1 mutation and conventional PCR for FLT3–ITD gene mutation analysis on 50 CN –AML patients. Twenty two (22/50)44% were NPM1 mutation positive only, (16/50) 32% were both NPM1 mutation and FLt3 positive and (18/50) 36% were FLT3–ITD positive only</p>

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Nada Tajuddin, ShadenMuawia, AmaniFouadSorour, Khalid Bassiouny Mohamed Detection of FLT3-ITD mutations and prognostic impact of NPM1 exon 12 mutations in adult CN-AML patients International Journal of Scientific Research, Vol : 4, Issue : 7 July 2015

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