Development of Protocol for Efficient  Clonal Propagation of Boerhaavia diffusa  L. Through in vitro Culture of Different  Explants

Arun Kumar; Pingal Kumari; Meenakshi Priyadarshni; Kumari Anjali; L.N. Shukla

Volume : II, Issue : VII, July - 2013

Development of Protocol for Efficient Clonal Propagation of Boerhaavia diffusa L. Through in vitro Culture of Different Explants

Arun Kumar, Pingal Kumari, Meenakshi Priyadarshni, Kumari Anjali, L. N. Shukla

Abstract :

Protocol for efficient clonal propagation of Boerhaavia diffusa L. through in vitro culture of different explants has been developed. Nodal segments and shoot tips were cultured on MS medium supplemented with, BAP, kinetin and NAA either alone or in combinations. Nodal segments when cultured for five weak on MS + 1.75mg/l BAP alone and along with 0.5mg/l KN gave 74% response for shoot induction. However, when MS medium was supplemented with 1.75mg/l BAP+ 0.5mg/l NAA, nodal segments produced similar result. It was observed that on the same medium the number of shoots was increased due to de novo development. MS medium supplemented with 1.75mg/l BAP+ 0.5mg/l IAA gave no better result. The highest average numbers of roots (18-20) were obtained on ½ MS +1.0mg/l IBA+1.0mg/l IAA. More than ninety percent of the cultures responded in rooting medium within 26 to 27 days of culture. The rooted plantlets were acclimatized in the laboratory conditions. Finally they were shifted to the field among which 75% survived.