Different Methods for Isolation of Bacteria in Burn Wound Infections with Its Antimicrobial Sensitivity Pattern: A Comparative Study in a Tertiary Care Hospital

Ritu Kumari; Tapashi Ghosh; Dibakar Haldar; Subhendu Bikash Saha; Jayanta Bikash De

Volume : VIII, Issue : V, May - 2019

Different Methods for Isolation of Bacteria in Burn Wound Infections with Its Antimicrobial Sensitivity Pattern: A Comparative Study in a Tertiary Care Hospital

Ritu Kumari, Tapashi Ghosh, Dibakar Haldar, Subhendu Bikash Saha, Jayanta Bikash De

Abstract :

Background: Burn wound sepsis is major cause of mortality. Objective: To explore method of isolation of bacteriological profile and antibiotic sensitivity for burn wound associated infection. Materials and Methods: A descriptive cohort study was conducted in 2018 involving 124 patients admitted in BSMCH with >20% total body surface area burn. Information was collected via interview, clinical examination and scrutinizing records using questionnaire. Wound swab, tissue biopsy and blood specimens were obtained from each patient for comparison efficacy in isolating bacteriae and their antibiotic sensitivity. Results: Majority (59.7%) were female patients belonged to the age group 21–30 years age sustaining burn between 20–40% TBSA with higher surface swab and tissue biopsy culture isolates. Blood culture positivity mostly was in 41–60% of TBSA burn. Swab and tissue culture positivity was significantly higher in each TBSA group compared to blood culture with significantly higher tissue biopsy and blood culture isolates across TBSA groups. High, moderate was noted between swab and tissue cultures for Pseudomonas aeruginosa; for Staphylococcus aureus, E. coli, Citrobacter freudii and Acinetobacter spp. Gram negative enterobacteriaceae were highly sensitive to Imipenem, piperacillin–tazobactam with high resistance towards cephalosporins and fluroquinolones. S. aureus exhibited highest (96%) sensitivity to vancomycin and linezolid whereas CoNS showed 100% sensitivity towards these two. Conclusion: This study suggested good correlation between surface swab and tissue biopsy for identifying pathogens on and within burn wounds