IJSR - International Journal of Scientific Research

Bonincontro A.; Domenici F.; Milardi G.L; Risuleo G.

Volume : VII, Issue : III, March - 2018

Differential dielectric behavior of the plasma membrane in mouse fibroblasts and human embryo kidney cells.

Bonincontro A. , Domenici F. , Milardi G. L, Risuleo G.

Abstract :

The dielectric features of “native” human emyo kidney cells (HEK293) and mouse fioblasts (3T6) were investigated by electrorotation. The specific dielectric parameters Capacitance (C) and Conductance (G) exhibit a significant difference between the two cell lines. In the case of the human one, both parameters result in an increase by one order of magnitude, in the early stage of the culture. This drastic difference with respect to 3T6 cells, tends to disappear as the culture time proceeds. Parameter C is directly related to the memane structure, while G mainly derives from the active and passive ion flux. The variation of these parameters is discussed in terms of an intrinsic functional/structural difference of the cell memane in the two cell lines. Finally, the phenomenon is not ascribable to memane mal–functions, as evidenced by cellular/molecular assays.