Volume : II, Issue : VII, July - 2013

Diversity of hydrolytic enzymes in Haloalkaliphilic archaea isolated from Lonar Lake

Jaysing Patil, Shyam Bajekal

Abstract :

Production of five hydrolytic enzymes was qualitatively studied in Haloalkaliphilic archaea isolated from Lonar Lake. All isolates were extreme haloalkaliphiles and were not able to grow under 15% salt and grew optimally at 20% with an optimum pH of 9.5. Phenotypic characteristics and 16S r-RNA sequence analysis showed the strains to be members of family Halobacteriaceae, belonging to genera Natrinema, Natrialba and Natronobacterium. All the three isolates were able to produce xylanase. Natrinema sp. produces amylase, caseinase, and cellulase where caseinase showed maximum activity at 23% salt and at 600 C at pH 9.5. Natrialba chahannaoensis produces amylase, caseinase, gelatinase and cellulase. Natronobacterium innermongoliae produces thermostable amylase with optimum temperature of 550 C and pH 9.0 and caseinase which showed maximum activity at 450 C and with 14% salt. The present investigation reports isolation and studies on the enzymatic diversity of extremely Haloalkaliphilic Archaea for biotechnological applications.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Jaysing Patil, Shyam Bajekal Diversity of hydrolytic enzymes in Haloalkaliphilic archaea isolated from Lonar Lake International Journal of Scientific Research, Vol : 2, Issue : 7 July 2013

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