DNA Profiling for Detection of Mixed  Semen Stain from Forensic Human Body  Stain using Y–STR Analysis

Unnati Solanki; Preeti Sharma; M. G. Shaikh; S. J. Nanawala

Volume : II, Issue : XI, November - 2013

DNA Profiling for Detection of Mixed Semen Stain from Forensic Human Body Stain using Y–STR Analysis

Unnati Solanki, Preeti Sharma, M. G. Shaikh, S. J. Nanawala

Abstract :

In sexual–assault cases, DNA profiling of mixed stains is a challenge, as the presence of a large quantity of female material that may prevent detection of the male DNA. Here investigation was undertaken for DNA profiling for forensic mixed semen stain of cases of sexual assault. An efficient method is differential extraction for isolating sperm DNA from a mixed fluid sample which contains vaginal epithelial cells in a greater amount. The modified method of the two–step differential extraction procedure was found to be suitable for separating sperms DNA and vaginal cell DNA from the mixed stains. In this study the forensic sample which is having mixture of male and female DNA, Y profiles were obtained using Y–STR analysis