DOWN REGULATED ABP1 (AMILORIDE BINDING PROTEIN) GENE EXPRESSION IN PHENYTOIN TREATED ALBINO RAT TESTIS–RT–PCR ANALYSIS

Rajkumar R; Vathsala Venkatesan

Volume : IV, Issue : I, January - 2015

DOWN REGULATED ABP1 (AMILORIDE BINDING PROTEIN) GENE EXPRESSION IN PHENYTOIN TREATED ALBINO RAT TESTIS–RT–PCR ANALYSIS

Rajkumar R, Vathsala Venkatesan

Abstract :

Phenytoin is used for grand malepilepsy, psychomotor, focal cortical, status epilepticus, trigeminal neuralgia, cardiac arrhythmia. Studies show that phenytoin directly affect ain regions that mediate sexuality. Phenytoin may cause sexual dysfunction by inducing secondary effects on reproductive hormones. The present study is aimed at the effect of phenytoin induced differential regulation ABP1 gene in albino rat testis .ABP1 (Amiloride binding protein) catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine Required for male and Female reproduction The albino rats were divided into two groups, control and test .The test group was given 120mgs/kg /body weight of phenytoin orally and equal amount of normal saline was given for the control group. After 45 days with the rat under deep anaesthesia ,the testis were removed from the scrotum and stored in liquid nitrogen .The stored specimens of testis of control and tests group were subjected to cDNA microarray analysis. This study showed the differential expression of gene ABP1 in test group when compared with the control group.