Effect of Administration of Lab Isolated L.Plantarum from GI Tract of Guinea Fowl on Intestinal Histomorphometry in Broilers

Vineetha P.G.; Simmi Tomar; Deepthi VijayK; Sreelekshmy Mohandas

Volume : III, Issue : VIII, August - 2014

Effect of Administration of Lab Isolated L.Plantarum from GI Tract of Guinea Fowl on Intestinal Histomorphometry in Broilers

Vineetha P. G. , Simmi Tomar, Deepthi Vijayk, Sreelekshmy Mohandas

Abstract :

The study was aimed to investigate the effect of feed supplements viz: Lactobacillus plantarum (lab isolate from gastrointestinal tract of Guinea fowl), Lactobacillus acidophilus (NCDC, Karnal) and in- feed antibiotic bacitracin methylene disalicylate (BMD) on intestinal histomorphometry in oilers. During the entire experimental period of 35 days in oilers, four treatment groups were provided with different dietary treatments (T1-basal diet (Control-1), T2-Antibiotic growth promoter BMD @ 20g /100kg feed (Control-2), T3 - 1x108cfu of L.acidophilus/gm fermented feed +MOS @ 1g /kg feed T4-1x108 cfu of lab isolated L.plantarum (L.I.L.p) /gm fermented feed+ MOS @ 1g /kg feed. There were 20 birds per each treatment group in Guinea fowl and 25 chicks in oilers. Histomorphological examination of duodenum, ileum of oilers fed different dietary treatments was conducted at 35th day of experiment. Six birds from each group were sacrificed and sections of intestine were cut and processed for histomorphological study and slides were prepared and examined using an optical microscope. In terms of histomorphology of duodenum was concerned, duodenal villous height (VH) and crypt depth (CD) was significantly higher for T4 and T3 and lowest values obtained for antibiotic fed T2 group. Whereas, ileal villous height didn�t show any significant difference among treatment groups. Ileal crypt depth was lowest for antibiotic supplemented T2 group over the other three groups. Duodenal villous height:crypt depth ratio was highest for T4 and T3 over T2 and control birds T1. The increment in the height of villi and villous height crypt depth ratio in Lactobacillus plantarum fed T4 group in oilers suggests that guinea fowl specific lab isolated L.plantarum may be used in commercial oiler production for improving growth by augmenting digestion and absorption of nutrients by modifying intestinal histomorphology