Effect of Buffer type and pH on  adsorption of Protein on Micro Particles  for development of Particle enhanced  Immunoassay

Pranav R. Naik; Dr. Pratibha B. Desai

Volume : II, Issue : VII, July - 2013

Effect of Buffer type and pH on adsorption of Protein on Micro Particles for development of Particle enhanced Immunoassay

Pranav R. Naik, Dr. Pratibha B. Desai

Abstract :

Latex particles coated with protein material have many applications in Biology and In Vitro Diagnosis. Particle Enhanced Immunoassay continues to be widely used for the detection of micro level and smaller quantity of Antigen or Antibody from various clinical samples. In present study Physical adsorption of Albumin and Globulin, two distinct protein types on to latex particle surface is analyzed with reference to buffer type and pH, major factor affecting adsorption. Study was carried out on different particle types, plain Polystyrene Micro particles (PS-MP) and Carboxyl modified Polystyrene Micro particles (CM-PS). We have observed trend of decreasing adsorption with increase pH. At pH 6.1 MES buffer gives maximum adsorption for both protein in all Micro Particles.