EFFECT OF N–ACETYLCYSTEIN (NAC) IN EXPERIMENTAL BLUNT LIVER INJURY

Edip Erdal YILMAZ; M.Hayri ERKOL; Nurettin KAHRAMANSOY; Aysel KUKNER; Tulin FIRAT; Mehmet TOSUN

Volume : VII, Issue : X, October - 2018

EFFECT OF N–ACETYLCYSTEIN (NAC) IN EXPERIMENTAL BLUNT LIVER INJURY

Edip Erdal Yilmaz, M. Hayri Erkol, Nurettin Kahramansoy, Aysel Kukner, Tulin Firat, Mehmet Tosun

Abstract :

Introduction and Aims:Nowadays, blunt liver injury is increasingly preferred to be managed non–operatively. Therefore, wound heal progressing agents are widely investigated. N–Acetylcystein (NAC), which has antioxidant effect, is an amino aside derivate and a glutathione precursor as well. Aim of this study is to investigate the effect of NAC at liver injured bluntly.

Materials and Methods:A total of 6 groups to be sham, NAC intraperitoneally (IP), and NAC intramuscular (IM) administrated6 rats in each group. These groups were separated for day 3 (Sham–3, IM–3 and İP–3) and day 7 (Sham–7, IM–7 and IP–7) terminations. Blunt abdominal trauma was applied to all groups. The dosage of NAC was 50mg/kg and 200mg/kg for intramuscular and intraperitoneal pathways respectively. Histopathological assessment was done by scoring inflammation severity between 0–3, apoptosis index(AI) by the method of tunnel and Ki–67 proliferation index (PI).

Results:AST and ALT levels were less in the seventh day groups than that of the third day groups. This decrease was significant in Sham–7 and IM–7 groups. Among the same day groups, AST, ALT and LDH levels were significantly low in IM and IP groups according to the Sham groups. However, there was no evident difference between IM and IP groups. Inflammation severity did not differ between the day 3 and 7 groups. But, it was significantly low in IM and IP groups compared to the Sham–3. Day 7 groups also showed difference. Ki–67 PI�sof the IM–3 and IP–3 groups were prominently higher than that of the IM–7 and IP–7 groups. AI, the seventh day of the third day, groups, IM and IP groups did not differerences significantly.AI groups‘ of the Sham–3, IM–3 groups were the differences between these groups were definite. Seventh–day AI groups, a significant differences between the sham group was higher in IM.

Conclusion:When all our results are examined, NAC is effective in the healing and regeneration of liver tissue after blunt liver trauma. However, further studies are required.