Volume : VII, Issue : VII, July - 2018

EFFECT OF SPECIMEN STORAGE TIME ON SERUM GLUCOSE ESTIMATION

Dr. Nitin Sharma, Siddharth Mundotiya, Dr. Ranjana Mathur

Abstract :

In rural areas of Jodhpur, due to inconsistent power supply or delay in transportation of specimen from the local collection centres to the clinical laboratory, early separation of serum from cells is not practically possible. The study aimed to analyze the impact of specimen storage time on serum glucose levels in healthy adults and to evaluate the pattern of change in glucose level from the time of collection in the laboratory up to 4 hours after collection.

 A total of 50 samples were analyzed. Glucose concentrations in Group – 1 serum samples (which were separated from clot / blood cells within 15 – 30 minutes of blood collection process) were consistently and significantly higher (129.68±12.45) when compared with the samples of Group – 2 (118.32±18.35) with a difference of 11.36 or 9.16 % (P = 0.0005), Group – 3 (111.62 ±11.77) with a difference of 18.06 or 14.97% (P <0.0001) and Group – 4 (105.88±14.93) with a difference of 23.8 or 20.21% (P <0.0001). We recommend that the health care providers must ensure quick delivery of samples for early separation of serum from cellular components.

We also recommend that if separation of serum within 15–30 minutes is possible, use of NaF fluoride tubes for glucose estimation during blood collection is not necessary. Constant evaluation of skills acquired by phlebotomists or nurses is required to check whether ISO guidelines are being followed.

Keywords :

Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Dr. Nitin Sharma, Siddharth Mundotiya, Dr. Ranjana Mathur, EFFECT OF SPECIMEN STORAGE TIME ON SERUM GLUCOSE ESTIMATION, INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : Volume-7 | Issue-7 | July-2018

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