Volume : V, Issue : II, February - 2016

Endothelial Nitric Oxide Synthase "eNOS" Gene Polymorphisms in Preeclampsia in South Indian Population.

Madhavi Puppala, Lakshmi Kalpana Veerathu, Anuradha A, Sudhakar Godi

Abstract :

 Introduction: Pregnancy is a hypercoagulable state with increased tendency for thrombus formation, a condition that is increased when combined with acquired or inherited risk factors that lead to thrombophilia. The association between endothelial nitric oxide synthase (eNOS) polymorphism, their haplotypes, serum nitric oxide (NO) levels and RPL, were studied in different ethnic populations. The results, however, were contradictory. Objective: This study was conducted in order to determine the association between promoter –786 T>C, exon 7 Glu298Asp (894 G>T) polymorphisms of eNOS gene in idiopathic Preeclampsia in women of Coastal Andhra Pradesh, Visakhapatnam, India. Materials and Methods: Our study included 45 (30 non–pregnant and 15 pregnant) women who suffered from unexplained preeclampsia, and 45 (30 non–pregnant and 15 pregnant) healthy women matched for age and without previous history of preeclampsia. DNA was extracted from whole blood samples. The PCR products of exon 7 Glu298Asp (894 G>T) and promoter –786T>C polymorphisms by PCR–RFLP, where they were digested using specific restriction enzymes and then separated electrophoretically using 2% agarose gel. Results: The C allele carrier which represented by (CC + CT) genotypes and the C allele of the promoter –786T>C polymorphism are significantly associated with increased risk of preeclampsia, where they presented with a higher frequency in this women. Neither exon 7 Glu298Asp(894G>T) was significantly associated with preeclampsia risk in the study population. Conclusion: The (CC + CT) genotypes (C allele carrier)and the C allele of the promoter –786T>C polymorphism are possible risk factors for preeclampsia. The study showed that the (C allele carrier) which represented by (CC + CT) genotypes is associated with preeclampsia.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Madhavi Puppala, Lakshmi Kalpana Veerathu, Anuradha A, Sudhakar Godi Endothelial Nitric Oxide Synthase "eNOS" Gene Polymorphisms in Preeclampsia in South Indian Population. International Journal of Scientific Research, Vol : 5, Issue : 2 February 2016

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