Volume : II, Issue : X, October - 2013

Epigenetic Changes of Brca1 and S100a4 Genes During Ovarian Cancer Progression

Akiko Horiuchi, Takuma Hayashi

Abstract :

Hypoxia is known to play important roles in the development and progression of tumors. We previously demonstrated that BRCA1 and S100A4, critical molecules for tumor initiation and metastasis, were differential expressed in ovarian cancer cells. Therefore, we examined the mechanisms of the BRCA1 and S100A4 expression in ovarian carcinoma cells, with particular attention paid to the effects of hypoxia. The expression levels of S100A4 and BRCA1 were found to correlate with the invasiveness of ovarian carcinoma cells in vitro and in vivo, and the BRCA1 and S100A4 expression was associated with epigenetic changes in the regulatory regions of BRCA1 or S100A4 cording genes in ovarian carcinoma cell lines and tissues. The expression of S100A4 was increased under hypoxia and was associated with elevated invasiveness. In addition, exposure to hypoxia reduced the methylation of hypoxia–response elements (HRE) of the S100A4 gene in a time–dependent fashion, in association with the increased binding of HIF–1?to a methylation–free HRE in ovarian carcinoma cells. These findings indicate that hypoxia–induced hypomethylation plays an essential role in S100A4 over expression and the epigenetic transformation of ovarian carcinoma cells into the ‘‘metastatic phenotype’’

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Akiko Horiuchi, Takuma Hayashi / Epigenetic Changes of Brca1 and S100a4 Genes During Ovarian Cancer Progression / International Journal of Scientific Research, Vol.2, Issue.10 October 2013

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