Estimation of rutin in ethanolic extract of  Brassica oleracea L. var capitata. leaves by  HPTLC method

Mohammed Fazil Ahmed; A. Srinivasa Rao

Volume : II, Issue : VIII, August - 2013

Estimation of rutin in ethanolic extract of Brassica oleracea L. var capitata. leaves by HPTLC method

Mohammed Fazil Ahmed, A. Srinivasa Rao

Abstract :

The aim of the study is to estimation biologically active flavonoidal compound, rutin, in ethanolic leaves extract of Brassica oleracea L. var capitata. Linn leaves by using high-performance thin-layer chromatography (HPTLC). Pre coated silica gel 60 F254 used as stationary phase and Toluene: Ethyl Acetate: methanol in ratio of 5: 3: 2 are used as mobile phase. Densitometric determination and quantification of this compound was carried out at 254 nm.The standard Rf values of rutin is found to be 0.17 . The total peak areas of the standards rutin were compared and the corresponding peak areas of extract were estimated to be 99.36 respectively. This HPTLC method was found to be simple and convenient for rapid screening of active compounds and quantification of the investigated flavonoid in Brassica oleracea L. var capitata. Linn.