Evaluating the Role of Mononuclear Cell Count in Predicting the Cell Dose of Apheresis Derived Peripheral Blood Stem Cells by Correlating with Flow Cytometry Based CD 34 Enumeration

Dr Bhushan Asthana; Dr Rounak Dubey; Dr Anantpreet Kaur; Dr Amit Pawar; Dr Amit Biswas

Volume : VII, Issue : XII, December - 2018

Evaluating the Role of Mononuclear Cell Count in Predicting the Cell Dose of Apheresis Derived Peripheral Blood Stem Cells by Correlating with Flow Cytometry Based CD 34 Enumeration

Dr Bhushan Asthana, Dr Rounak Dubey, Dr Anantpreet Kaur, Dr Amit Pawar, Dr Amit Biswas

Abstract :

The high cost associated with the Peripheral Blood Stem Cell (PBSC) transplants still remains a major concern and any possible measure to mitigate the financial burden is always welcome. The engraftment of transplanted PBSCs is predicted by the enumeration of CD34+ cells, which is carried out multiple times before, during and after the PBSC harvest procedure. The study included 107 consecutive apheresis procedures performed on donors who were sent to our centre for PBSC harvest. Correlations between the number of CD34+ cells and MNC in the apheresis products were assessed using Karl Pearson�s Coefficient of Correlation. The study group was divided into two categories, comprising of allogenic harvests and autologous harvests. The correlation between the stem cell product yield calculated using MNC count and CD34 count was found to be moderately significant (0.673) in case of allogenic but no statistically significant coefficient of correlation could be observed in case of autologous donors. The findings of this study suggest that obtaining the MNC count can be employed as a useful and cost–effective method of predicting the yield during the mid–cycle of the PBSC harvest procedure in case of an allogenic donor