EVALUATION OF ANTIBACTERIAL EFFICACY OF 2% CHLORHEXIDINE GEL, CALCIUM HYDROXIDE AND LINCOSAMIDE BASED ANTIBIOTIC AGAINST ENTEROCOCCUS FAECALIS– AN IN VITRO STUDY

Dr L.R.SURENDER; Dr Bhasker Bhamini; Dr C. Rakesh Reddy; Dr D.sainath; Dr Naresh Kumar. K; Dr Sneha Pallepagu

Volume : VIII, Issue : V, May - 2019

EVALUATION OF ANTIBACTERIAL EFFICACY OF 2% CHLORHEXIDINE GEL, CALCIUM HYDROXIDE AND LINCOSAMIDE BASED ANTIBIOTIC AGAINST ENTEROCOCCUS FAECALIS– AN IN VITRO STUDY

Dr L. R. Surender, Dr Bhasker Bhamini, Dr C. Rakesh Reddy, Dr D. Sainath, Dr Naresh Kumar. K, Dr Sneha Pallepagu

Abstract :

AIM: Aim of the present study is to evaluate the antibacterial efficacy of 2% Chlorhexidine gel (CHX), calcium hydroxide (CH), Clindamycin 1%w/v (CLI), and combination of Clindamycin 1%w/v (CLI) with Calcium Hydroxide (CH) against Enterococcus faecalis (EF). MATERIALS AND METHODS: Enterococcus faecalis strains were mixed with peptone water and the turbidity was adjusted equal to the McFarland�s turbidity standard tube No: 0.5. The inoculum obtained were used to make lawn cultures on the agar plates. A total of 30 agar plates were prepared, with each plate having four wells containing the four medicaments. The plates were incubated and evaluated for zones of inhibition after 24 hours. The results were statistically evaluated by paired t–test, ANOVA and Post–hoc analysis using Turkey�s HSD. RESULTS: The difference between values of the zones of inhibition around each medicaments after 24 hours was found to be statistically significant. The maximum mean value of the zones of inhibition after 24 hours was shown by group I CHX (11.19 � 0.75), while the minimum was shown by group II CH (6.03� 0.67). CONCLUSION: Within the confines of the study, it can be concluded that clindamycin can be used as an alternate to calcium hydroxide dressing as an intracanal medicament. Combination of calcium hydroxide and clindamycin showed synergistic action as proven in the present study.