Evaluation of Catridge based Nucleic Acid Amplification Test in Diagnosis of Tuberculosis in patients attending to Government General Hospital, Ananthapuramu

Meeniga Sailaja; Poojari Srinivasulu Naik; Gundela Swarnalatha; Sudheer Babu

Volume : IX, Issue : VI, June - 2020

Evaluation of Catridge based Nucleic Acid Amplification Test in Diagnosis of Tuberculosis in patients attending to Government General Hospital, Ananthapuramu

Meeniga Sailaja, Poojari Srinivasulu Naik, Gundela Swarnalatha, Sudheer Babu

Abstract :

Background: Diagnosis of Pulmonary tuberculosis is mostly relied on sputum smear microscopy and sputum for mycobacterium culture and chest radiographs. In recent years, CBNAAT has been recommended by World Health Organization diagnostic test. Materials and Methods: All retreated cases of Pulmonary and extra pulmonary TB, newly detected smear–negative and all PTB–HIV cases were recruited for the study and sputum for CBNAAT was performed in all of them. Results: Out of 2561 suspected cases of TB, 447 were sputum smear negative and 2114 cases were sputum smear positive. Overall Sensitivity of CBNAAT was 64%, Specificity 94%, PPV and NPV 70% and 87% respectively. TB–HIV co–infected patients attributed to 9.36%. 95.36% were Rifampicin sensitive, 3.96% were resistance & 0.67% were rifampicin intermediate sensitive. Conclusion: CBNAAT is found to be better diagnostic method and has an additional advantage of identifying rifampicin resistance with high sensitivity and specificity.