Evaluation of in vitro Antioxidant and Free Radical Scavenging Properties of Different Parts of Medicinal Plant Nothapodytes foetida (Family: Icacinaceaae)

Kailas D. Datkhile; Pratik P Durgawale; Madhavi N. Patil; Rohit D. Vhaval; Tejasvi S. Khamkar

Volume : V, Issue : V, May - 2016

Evaluation of in vitro Antioxidant and Free Radical Scavenging Properties of Different Parts of Medicinal Plant Nothapodytes foetida (Family: Icacinaceaae)

Kailas D. Datkhile, Pratik P Durgawale, Madhavi N. Patil, Rohit D. Vhaval, Tejasvi S. Khamkar

Abstract :

This study was designed to examine the in vitro antioxidant properties of different parts (leaves, stem, bark, fruits and seeds) of Nothapodytes foetida, which is a major source of anticancer drug, camptothecin. Antioxidant activites of the aqueous and methanolic extracts have been evaluated by various in vitro models. In vitro antioxidant activity was evaluated by percentage inhibition in different assays including 2, 2�–diphenyl–1–picrylhydrazyl (DPPH), (2, 2�–azinobis–(3–ethylbenzothiazoline–6–sulfonic acid (ABTS•+), hydroxyl radical scavenging assay, superoxide radical scavenging assays and reducing power capacity. The results obtained from the present study indicated that the N. foetida leaf, fruit and seed extract is a potent source of natural antioxidants and both the aqueous and methanolic extracts possesses excellent anti–oxidant and free radical scavenging activity. It was also observed that the leaves, fruits and seeds of N. foetida possessed potent free radical scavenging ability as compared to stem and bark. In all the assays performed, aqueous extract was found to possess higher antioxidant activity than methanolic extracts.