Volume : II, Issue : IV, April - 2013

Evaluation of Phosphate Solubilizing Activity and Indole Acetic Acid Production of Rhizobia

Abhi Garg, Manoj Sharma

Abstract :

Rhizobia are a group of soil bacteria that fix nitrogen symbiotically with legumes.They are gram negative, motile rod shaped bacteria. In this study, attempts have been made to evaluate the potential of Rhizobium to be used as phosphate solubilizing bacteria along with indole acetic acid production. Ajmer being a semi arid zone of Rajasthan was selected for the study of these phosphate solublizer and indole acetic acid producer isolated from root nodules of Trigonella foenumgraecum and Tephrosia purpurea .Twenty bacterial forms were isolated and screened . Phosphate solubilization was detected both quantitatively as well as qualitatively and effects of Tryptophan on production of Indole acetic acid and the optimal incubation period were also determined. IAA produced by the Rhizobium could be used as sprays for the substitute of growth hormones produced by plants .Sixteen strains were found positive for phosphate solubilization and fourteen were positive for Indole acetic acid production with 150 µg/ml of Tryptophan for 72 hrs of incubation period. Thus, the potential capacity of present Rhizobial isolate in phosphate solubilization and Indole acetic acid production can be used as biological fertilizers and would play significant role in achieving sustainable agriculture and also maintain the eco-friendly environment as a substitute of chemical fertilizers . Application of such strains in the form of biofertilizers to nutrient deficient soil could be used as promising mode for the enrichment of nutrient deficient soils

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Article: Download PDF   DOI : 10.36106/ijsr  

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Abhi Garg,Manoj Sharma Evaluation of Phosphate Solubilizing Activity and Indole Acetic Acid Production of Rhizobia International Journal of Scientific Research, Vol.II, Issue.IV April 2013

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