Evaluation of the cytotoxic effects of Helicia nilagirica Bedd in vitro

Jennifer Zoremsiami; Ganesh Chandra Jagetia

Volume : VI, Issue : IX, September - 2017

Evaluation of the cytotoxic effects of Helicia nilagirica Bedd in vitro

Jennifer Zoremsiami, Ganesh Chandra Jagetia

Abstract :

The cancer is a second largest killer disease and despite numerous advances made in the treatment strategies, the complete cure of cancer remains elusive. Therefore, the present study was undertaken to investigate the anticancer potential of aqueous extract of Helicia nilagirica (HNA) in vitro by MTT and clonogenic assays, where V79 and HeLa cells were treated with the different concentrations of aqueous extract of Helicia nilagirica. The treatment of V79 and HeLa cells with HNA resulted in a concentration dependent increase in cytotoxicity, which was maximum at the highest concentration of 400 �g/ml HNA in both the cell lines. The results of MTT assay were further confirmed by clonogenic assay, which also showed a concentration dependent decrease in the clonogenicity of HeLa cells.. To understand the mechanism of action the effect of HNA on glutathione (GSH) concentration, activities of glutathione–s–transferase (GST), catalase and superoxide dismutase (SOD) were studied at different post HNA treatment times. The exposure of HeLa cells to different concentrations of HNA at different post– treatment time alleviated the GSH content and also reduced the activities of antioxidant GST,CAT and SOD in a concentration and time dependent manner, except GST which was lowest and 6 h post–treatment and then marginally elevated at 12 h post–treatment.. The present study indicates that HNA exerted the cytotoxic effect on HeLa cells and recued the cell survival and this effect of HNA may be due to the alleviated level of the GSH, GST, catalase and SOD.