Evaluation of The Nucleolar Organizer Region Associated Proteins In Odontogenic Keratocyst And The Role Of AgNORs In Cell Proliferation: A research analysis

Dr. Jazib Nazeer; Dr.Md Asad Iqubal; Dr Rohit Singh

Volume : VIII, Issue : XII, December - 2019

Evaluation of The Nucleolar Organizer Region Associated Proteins In Odontogenic Keratocyst And The Role Of AgNORs In Cell Proliferation: A research analysis

Dr. Jazib Nazeer, Dr. Md Asad Iqubal, Dr Rohit Singh

Abstract :

Objective– the main objective of the current study is To assess and determine the clinical and pathological features of OKC, To compare the clinical and pathological characteristics of inflamed OKCs with the non– inflamed ones and To determine the proliferative capacity of the basal and parabasal layers of both inflamed and non– inflamed OKC lining by counting AgNORs. Material and method– 20 cases of histologically proven OKC paraffin blocks were taken from the archive, and were divided into two groups and are subsequently stained with Haematoxylin and Eosin and AgNOR. AgNOR staining technique was adapted from that described by Crocker et al. Result– 90% of cases (equal number of both) showed parakeratinized epithelium. Interestingly, varying thi ckness of epithelium was found in 23.30 % of the case and all of them were of inflamed type. In these cases, rete peg formation was also noted. The cells in the basal layer was cuboidal in 53.30 % of cases. Majority of the cysts (63.30%) had the typical corrugated surface. Conclusion– A statistically significant association was found between the presence of inflammation in the cyst wall and increase in thickness of the lining, rete peg formation and presence of odontogenic epithelial islands. The AgNOR counts were statistically significant in the inflamed OKCs as compared to non – inflamed OKCs. This study implicates that there is a greater chance of recurrent cyst occurring in inflamed OKCs when compared to the non–inflamed ones