Evaluation of Three Approaches For  The Measurement of Rna Integrity,  Concentration and Purity in Tissues of  Apricot Flower Buds

Ales Eichmeier; Tomas Kiss; Jana Cechova

Volume : III, Issue : III, March - 2014

Evaluation of Three Approaches For The Measurement of Rna Integrity, Concentration and Purity in Tissues of Apricot Flower Buds

Ales Eichmeier, Tomas Kiss, Jana Cechova

Abstract :

The RNA quantity and quality are basic factors for successful studies of gene expression. On absorbance and fluorescence based measurements are routinely used techniques for RNA measurement. A new technique for measurement of RNA is available with Agilent 2100 bioanalyzer in combination with the RNA 6000 Nano LabChip� kit. This method offers in addition the advantage of assessing RNA integrity. Agilent 2100 Bioanalyzer is compared with UV spectrophotometry and fluorometry quantitation systems assessing the quantity, purity and RNA integrity. This study was focused on comparison of RNA isolated from apricot flower buds which were evaluated by mentioned methods. Obtained results show important differences in RNA which was isolated in 2008 (stored at –80 oC) and RNA which was newly isolated from the same stored tissues in 2013. Study also compares techniques of mRNA quantity, quality and integrity evaluation.