EVALUATION OF TISSUE FIXATION USING ORGANIC SUBSTITUTES - A COMPARATIVE STUDY

SUDHA GUNASEKARAN; JAYANT.V.S; KARUNYA KRISHNAKUMAR; ASHWINI AJAY

Volume : VIII, Issue : III, March - 2019

EVALUATION OF TISSUE FIXATION USING ORGANIC SUBSTITUTES - A COMPARATIVE STUDY

Sudha Gunasekaran, Jayant. V. S, Karunya Krishnakumar, Ashwini Ajay

Abstract :

Fixation of tissue is an initial and important step in tissue processing in microscopical examination. It is a critical step in the preparation of tissues. Fixation is considered to preserve the tissues in a life –like state, prevent bacterial putrefaction, prevent autolysis and to increase the refractive index of the tissue. The present study used a natural fixative instead of chemicals in order to prevent the deleterious effects of the chemical fixatives. The aim of the study is to compare the tissue fixation ability of Apple cider vinegar and Honey Lemon Ginger Tea extract with that of formalin using H and E stain and to determine the best fixation among the two. Tissue section were assessed for cytoplasmic, nuclear details and staining quality under light microscope. Results were analysed by Kruskal Wallis ANOVA .Preservation of tissue by Apple cider vinegar gave good results than honey ginger lemon tea after comp� with formalin.