IJSR - International Journal of Scientific Research

Anju Sebastian; Mercy John Idikula

Volume : V, Issue : VI, June - 2016

Excretory– secretory proteins of Mycobacterium tuberculosis strain H37Rv and local clinical isolates– A comparison

Anju Sebastian, Mercy John Idikula

Abstract :

 Proteins that are secreted actively by Mycobacterium tuberculosis in invitro cultures (ES proteins) are said to be equivalent to those released in vivo. The ES proteins have been reported to be immunodominant. In this study, a comparison of the ES proteins of the prototype strain H37Rv with that of local clinical isolates has been done by One dimensional Polyacrylamide gel electrophoresis. A thick sharp band was observed in the region between 66kDa and 44kDa in the PAGE of all clinical isolates, which was not visible for H37Rv. The salient antigens from clinical isolates of Mycobacterium tuberculosis associated with active disease may substantially improve the presently available diagnostic tools and may be explored for improving the efficacy of vaccines.