Explanatum explanatum: effect on viability and histology following in vitro treatment with phenyl vinyl sulfone

Chandra Mohan; Masoodul Haque; S. Nirjhar Chandra Ray; Afsana Begum

Volume : V, Issue : II, February - 2016

Explanatum explanatum: effect on viability and histology following in vitro treatment with phenyl vinyl sulfone

Chandra Mohan, Masoodul Haque, S. Nirjhar Chandra Ray, Afsana Begum

Abstract :

The objective was to study the in vitro effect of the antihelminthic drug phenyl vinyl sulfone (PVS) on the trematode Explanatum explanatum (Creplin, 1847) Fukui, 1929; during different periods of incubation. Trematodes infect liver of buffaloes and other cattle and cause extensive damage and have wide geographical distribution in India and other Asian countries. The drug phenyl vinyl sulfone (PVS) is known to have antihelminthic and antiprotozoal properties because it inhibits cysteine protease activities in parasite. Adult E. explanatum obtained from the liver of slaughtered buffaloes were treated with PVS in RPMI culture media and incubated at 37oC for periods ranging from 2 to 8 h. The effect of this protease inhibitor on worms was analyzed in terms of viability, motility and death of worms as well as histological changes. The minimum effective concentration of the drug was found to be 50 ppm after 8 h of incubation. 200 ppm concentration of the drug resulted in immediate death of adult flukes in vitro. Histological studies of the treated fluke revealed structural changes in the tegument epidermis and loss of spine. Degenerative changes were also noticed in the testicular follicles, ovaries and vitellaria when compared with untreated controlled group. The results suggest the PVS affects the mobility and histology of the trematode and could also be explored for use as an alternative antihelminthic drug