EXPRESSION STUDY OF INTERLEUKIN 8 IN DEONI CATTLE BY REAL TIME POLYMERASE CHAIN REACTION (RT–PCR)

Sonali Thakur; Soumyalekshmi; B. R. Shome; D.N. Das

Volume : V, Issue : I, January - 2016

EXPRESSION STUDY OF INTERLEUKIN 8 IN DEONI CATTLE BY REAL TIME POLYMERASE CHAIN REACTION (RT–PCR)

Sonali Thakur, Soumyalekshmi, B. R. Shome, D. N. Das

Abstract :

Interleukin– 8 (IL 8), also known as Neutrophil Activator Agent, is a chemokine molecule involved in the activation and extravasation of neutrophils during an inflammatory response. A remarkable property of IL 8 is its variable expression in different cells under different conditions. In the present study, quantitative real time PCR was used to analyse the expression of IL 8 gene in milk somatic cells from subclinical mastitic cows in relation to healthy cows of Deoni eed. It was found that IL 8 expression was significantly increased in response to subclinical mastitis. The change in the expression profile of IL 8 in subclinical mastitis shows the significant role played by this cytokine in mediating the inflammatory reactions during mastitis in Deoni cattle. This study reveals the importance of IL 8 as valuable biomarker of subclinical mastitis and the scope of manipulation of IL 8 expression in controlling mastitis in cattle.