Extracellular Alkaline Protease from a  Newly Isolated Bacillus stratosphericus  DF: Isolation and Identification

D. R. Aruna Bindu; I. Bhaskar Reddy

Volume : II, Issue : VI, June - 2013

Extracellular Alkaline Protease from a Newly Isolated Bacillus stratosphericus DF: Isolation and Identification

D. R. Aruna Bindu, I. Bhaskar Reddy

Abstract :

Bacterial extracellular alkaline proteases are of great importance due to its wide spectrum applications in detergent industries, bioremediation, food industries, and leather processing and bio-film degradation. The aim of the current study was to isolate protease producing bacteria from the soil sample collected at dump yard of dairy form industry, Vishakhapatnam. Total 60 bacterial colonies were isolated from the collected soil sample and all the bacterial strains were purified and tested for proteolytic activity. Out of these, one isolate named DF was selected on the basis of maximum hydrolysis for further study and it was also found to produce the highest yield quantitatively (492 U/ml). The selected strain DF was identified as Bacillus stratosphericus on the basis of morphology, biochemical tests and 16S rRNA sequence analysis. The present study is the first report to examine and describe production of alkaline protease from Bacillus stratosphericus.