Extraction of Flavonoids and Tannins in   Roots of Derris Trifoliata L and Evaluation for Antibacterial and Antioxidant Potentials

N. SHARIEF MD; A. SRINIVASULU; Uma Maheswara Rao V

Volume : III, Issue : V, May - 2014

Extraction of Flavonoids and Tannins in Roots of Derris Trifoliata L and Evaluation for Antibacterial and Antioxidant Potentials

N. Sharief Md, A. Srinivasulu, Uma Maheswara Rao V

Abstract :

The plant extracts of Derris trifoliata L finds a prominent place in folk medicine. In this study, we extracted and quantified flavonoids and tannin from the roots of Derris trifoliata in ethyl acetate and methanol, them evaluated their antioxidant activity by FRAP assay and antibacterial activity by agar well diffusion method. The bacterial species are Enterobacter aerogenes , Enterobacter cloacae, Staphylococcus aureus and Streptococcus pyogenes were compared with standard antibiotic gentamicin. The Minimum Inhibitory Concentration [MIC] was determined by serial dilution method. The methanol extracts showed highest levels of antioxidant activity as well as the inhibitory effect on the test cultures used. The MIC value was found to be between 2.5 to 5mg/100�l. This study provides the necessary data for extraction and characterization of bioactive principles that possess the antioxidant and antibacterial action from ethyl acetate and methanol root extracts of Derris trifoliata