Volume : IV, Issue : I, January - 2015

Extration And Qualitative Analysis of Peptide Hormone in A Crustacean

T. Saritha

Abstract :

Eye stalk ablation is the most common procedure used to induce crustacean reproduction in commercial hatcheries. How ever, other physiological and metabolic process is affected by the removal of the X- organ sinus gland complex located in the eyestalk. Macro achium rosenbergii is a commercially important species of crustacean cultured extensively throughout Southeast Asia. The main objective of this experiment was extraction and qualitative estimation of MIH hormones. The easiest way of extraction of proteins can be done by “Salt Precipitation method”. Fraction-I, II and III of the samples have shown the presence of proteins. The samples are having proteins with different molecular weight ranging from 7-14 kd. Salt precipitate and MIH specific-antibody clearly showed that MIH is thoroughly extracted from the eye stalks through salt-precipitate without the necessity of cost devices such as HPLC at least at the preliminary phases of this investigation.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

T.Saritha Extration And Qualitative Analysis of Peptide Hormone in A Crustacean International Journal of Scientific Research, Vol : 4, Issue : 1 January 2015

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