Genetic Variation in Yellowfin Tuna  Thunnus Albacares (Bonnaterre, 1788)  Along Indian Coast Using Pcr–Rflp Analysis  of Mitochondrial Dna D–Loop Region

Swaraj Priyaranjan Kunal; Girish Kumar; Maria Rosalia Menezes

Volume : III, Issue : I, January - 2014

Genetic Variation in Yellowfin Tuna Thunnus Albacares (Bonnaterre, 1788) Along Indian Coast Using Pcr–Rflp Analysis of Mitochondrial Dna D–Loop Region

Swaraj Priyaranjan Kunal, Girish Kumar, Maria Rosalia Menezes

Abstract :

Yellowfin tuna Thunnus albacares is an epipelagic, oceanic species of family Scomidae, found in tropical and subtropical region of the Pacific, Atlantic and the Indian Ocean. It is a commercially important fish species. In present study, population structure of yellowfin tuna was examined using PCR-RFLP analyses of mitochondrial DNA D-loop region from eight geographically distinct locations along the Indian coast. A 500 bp segment of D-loop region was analysed for 370 yellowfin samples using six restriction enzymes (Alu I, Hae III, Hinf I, Hha I, Msp I and Rsa I), resulting in 14 composite mitotype. Analysis of molecular variance (AMOVA) showed no significant genetic differentiation among sampling localities (ΦST = 0.00185; P= 0.327). Thus finding of this study suggests single panmictic population of yellowfin tuna in Indian waters.