Volume : II, Issue : VII, July - 2013

Genomic Dna Isolation From Plumbago L. Species Growing In India

Varsha N. Nathar, Prashant J. Gadge

Abstract :

Plumbago L. is a genus belonging to family plumbaginaceae consists of twelve species of which, three species P. zeylanica L., P. auriculata Lam., and P. rosea L. occur mostly in warm temperate to tropical regions of the India. The genus Plumbago is highly medicinal and has high amount of phenolic content i.e. plumbagin or 2-4 napthoquinone. The present study aims at standardization of DNA isolation protocol from leaves of all the three species i.e. P. zeylanica, P. auriculata, and P. rosea. The DNA isolation was performed by CTAB method without liquid nitrogen and some modifications. The DNA was purified and quantified comparatively in all the three species. The purification absorbance values obtained were 1.76, 1.82, and 1.86 for P. rosea, P. zeylanica and P. auriculata respectively, DNA concentration was calculated as 4.40, 4.55 and 4.65 µg/µl. This DNA was compared with the DNA isolated by standard method of Doyle J.J. and Doyle J.L. (1987). The pure isolated DNA is used for further molecular studies and the focus is on genome characterization of the three species using molecular markers RAPD, RFLP and SSR.

Keywords :

Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Varsha N. Nathar, Prashant J. Gadge Genomic Dna Isolation From Plumbago L. Species Growing In India International Journal of Scientific Research, Vol : 2, Issue : 7 July 2013

Number of Downloads : 1161

References :