Haematological and Immunophenotypical Study on Acute Leukemias in a Tertiary Care Centre

Bidhan Ch. Das; Upam Kr. Sharma; Vishal Agarwal

Volume : VI, Issue : V, May - 2017

Haematological and Immunophenotypical Study on Acute Leukemias in a Tertiary Care Centre

Bidhan Ch. Das, Upam Kr. Sharma, Vishal Agarwal

Abstract :

Acute leukemias are heterogeneous group of haematological malignancies characterized by clonal expansion of immature myeloid or lymphoid precursors (blasts). The present study was conducted to have haematological as well as immunophenotypical interpretation in the diagnosis of acute leukemia. This study was performed over a peroid of 1 year from April 2016 to March 2017. The complete haemograms were done and slides were prepared with peripheral blood and in selected cases bone marrow aspirates which were stained by leishman and giemsa stain. Flow cytometric immunophenotyping was done to establish the types of acute leukemias. In our study, 52 cases of acute leukemias were reported. After confirmation by immunophenotyping, there were total 59.6% cases of AML, 28.8% of B–cell ALL, 5.7% of T–cell ALL, 3.8% of bi–lineage and 1.9% cases of bi–phenotypic leukemia. It is imperative to ascertain the lineage of leukemia by both morphological features and immunophenotyping. Immunophenotyping is a sensitive means to monitor the progress of patients after chemotherapy, risk stratification and aids in detection of minimal residual disease.